Table 2.

CRISPR editing events in CH12F3 as revealed by deep sequencing

#	Subclone	Target residues	Indels	Mut	% of total reads
1	Ung1.4-B2	1 (M)	–10	-	91.1
2	Ung1.5-B2	13 (R)	–2	-	83.1
3	Ung1.5-B9	13 (R)/10 (R)	–2/–52	-	49.2/38.6
1	Ung2.1-A2	33 (A)/35 (E)	–10/–10	-	42.1/39.3
2	Ung2.1-A9	36 (I)	+1	-	78.2
3	Ung2.1-A28	35 (E)	–13	-	88.4
1	Ung1+2-A6	87 (F)/88 (G)	+32/–26	3/0	45.8/30.6
2	Ung1+2-B1	88 (G)/85 (A)	–10/+1	0/8	44.1/38.0
3	Ung1+2-B3	89 (E)	–2	-	85.8
1	Int2-A5	-	–4/–9	0/1	45.6/41.4
2	Int2-A6	-	0/–8	-	41.9/41.2
3	Int2-B1	-	–5/–4	1/1	45.5/39.9
1	Aicda-B11	23 (G)/23 (G)	–1/–5	-	48.7/46.2
2	Aicda-B39	23 (G)	–1	-	94.2
3	Aicda-B47	22 (K)	–3+1	2	94.3

Indels: Insertions (+) or deletions (–). Mut: mutations. Int2: Ung intron 2 (mock control). Differently edited alleles are separated by ‘/’.