Figure 2.

MALDI-TOF mass spectrometric analysis of aminoacylation of 3′-NH₂-tRNA catalyzed by flexizymes. (A) MALDI-TOF mass spectra of RNase T1-treated 3′-aminoacyl-NH-tRNA fragments, either charged by eFx or dFx. Red arrows indicate the peaks of the fragments derived from aminoacyl-tRNA, and black arrows are those of non-acylated ones. Calculated (Calcd.) and observed (Obsd.) m/z values of the [M+H]⁺ ions are also shown. * and † indicate the [M+Na]⁺ and [M+K]⁺ ions, respectively. (B) Ratios of the peak intensities of MALDI-TOF mass spectra shown in (A). Values are calculated by the intensity of the acylated peak divided by the sum of non-acylated and acylated peak; n = 3, error bars; s.d.