Figure 4.

Tryptophan at position 45 is required for proper biogenesis of HIV-1 mRNA species and viral production. (A) Left. Three different primer pairs were used to amplify alternatively spliced mRNAs produced during HIV-1 life cycle: fully spliced, single spliced and unspliced transcripts. Right. HeLafB cells expressing the Rev W45A mutant show increased levels of single spliced mRNAs compared to cells expressing Rev WT, as measured by RT-PCR. (B) Supernatant of HeLafB cells expressing the Rev W45A mutant show reduced p24 levels compared to cells expressing Rev WT, as measured by p24 ELISA.