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. 2019 Mar 27;47(9):4521–4538. doi: 10.1093/nar/gkz178

Figure 3.

Figure 3.

HDAC1 knockdown or TSA treatment increases KDM5A occupancy on mitotic chromatin at sites containing the RBPJ-binding consensus sequence. (A) Experimental scheme. Asynchronous (A) and mitotic (M) cells were collected under the indicated experimental conditions and subjected to KDM5A ChIP. (B, C) KDM5A ChIP-qPCR analyses at RBPJ binding sites that contain the RBPJ-binding motif. (D, E) KDM5A ChIP-qPCR analyses at RBPJ binding sites that do not contain the RBPJ-binding motif. (F) Control KDM5A ChIP assays as in panels B and D analysing KDM5A enrichment at a KDM5A nonbinding region (NB3). (G) Control KDM5A ChIP assays as in panels C and E analysing KDM5A enrichment at a KDM5A nonbinding region (NB3). Shown are means ± SEM from two biological replicates. Paired t-tests were performed to compare enrichment in treated relative to untreated cells. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.