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Strategy for reconstructing Fab clones from NGS information. CDR strips (L3-H3 or L3-H1-H2-H3) generated from round-3 phage pools are subjected to Ion Torrent Sequencing. Following NGS analysis, desired CDR combinations are reconstructed by cloning CDR-encoding oligonucleotides into the Hu4D5 Fab-encoding template phagemid by Kunkel mutagenesis.
