Figure 4.

T181 is required for full IRF1 transactivation of target genes. (A) IRF1 target gene mRNA expression determined by qRT-PCR. H3396-Tet-Off cells expressing empty vector (pCDNA4-TO), WT or T181A IRF1, were induced with 2 μg/ml Dox for 36 hr. TRAIL, OAS3, PSMA6 and TBC1D32 mRNA is expressed relative to β-actin. Statistical significance was determined between Dox-induced WT and T181A IRF1 expressing cells. (B) ChIP analysis performed on the cell lines from (A) using either control rabbit IgG antibody or IRF1 M20 (mouse-specific) antibody to prevent any endogenous IRF1 immunoprecipitation. Data is shown as fold enrichment between cells treated with Dox (36 h 2 μg/ml) or vehicle.