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. 2019 Mar 30;47(9):4375–4392. doi: 10.1093/nar/gkz214

Figure 3.

Figure 3.

Uptake and intracellular distribution of additional ASO molecules across NSCLC cell lines (A) AZD4785 and Ionis 746275-binding sites on KRAS mRNA. (B and C) NSCLC cell lines were treated with a dose range of (B) Ionis 746275 or (C) Ionis 549148 for 72 h and RT-qPCR used to assess KRAS mRNA levels. Data were normalized to housekeeping gene 18S rRNA and to PBS control. Data shown are representative of multiple experiments. (D and E) Cell lines were seeded onto glass-bottomed plates and treated with 10 μM of (D) Ionis 746275 or (E) Ionis 549148. Cells were fixed at 72 h and nuclei stained with Hoechst, cytoskeleton with alpha-tubulin and ASO with anti-ASO antibody, and images were processed using FIJI (ImageJ) software; scale bar is 10 μm.