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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Mol Cancer Res. 2018 Jun 22;16(10):1579–1589. doi: 10.1158/1541-7786.MCR-18-0151

Figure 5. Functionalized fibronectin fibrils facilitate EMT and directional cellular migration.

Figure 5.

(A) Control (scram) and β1 integrin depleted (shβ1 Int) Ca1a cells were cultured on fibronectin coated 3D scaffolds as described in the methods for 6 days and images were acquired using phase contrast microscopy. Arrows indicate single cells (scram) or cell clusters (shβ1 Int) growing off of the solid support of the scaffold. (B and C) The cells cultured as described in panel A were stained with antibodies against Ecad in panel B or phospho-STAT3 in panel C. In both cases cells were counter stained with phalloidin (red) and dapi (blue) to visualize the actin cytoskeleton and the nucleus, respectively. (D) Control (scram) and β1 integrin depleted (shβ1 Int) Ca1a cells were cultured on fibronectin coated 3D scaffolds or polystyrene (PS) for 6 days and analyzed by flow cytometry for cell surface expression of CD44 and CD24.