Figure 3.

Effects of SHINBARO on inflammatory mediators in the LSS rat model. (a) Serum was collected from rats with LSS and was analyzed for PGE₂ levels using an ELISA kit. The data are expressed as the mean ± SD (n = 4). (b) The mRNA expression of iNOS and COX-2 in the spinal cord was determined by real-time RT-PCR. The results were normalized using β-actin as an internal control. The data are expressed as the mean ± SD (n = 4). (c) The protein expression of iNOS and COX-2 in spinal cord tissues was determined by western blot analysis. β-Actin was used as an internal control. Relative intensity of indicated proteins was semiquantified using the NIH ImageJ software.