Figure 5.

The effect of UCP2 knockdown on LPS-induced activation of MAPK. Cardiomyocytes were transduced with Ad-shRNA-NC or Ad-shRNA-UCP2 adenovirus followed by treatment with or without 25 μM LPS. (a) Effects of UCP2 downregulation on the protein expression of total and phosphorylated p38 were assessed by western blotting. (b) Quantification relative to GAPDH level. Data were collected from three individual experiments and presented as the mean ± SD. ^∗ P < 0.05 vs. shRNA-NC, ^# P < 0.05 vs. shRNA-NC+LPS.