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. 2019 Apr 18;8(9):e011856. doi: 10.1161/JAHA.118.011856

Figure 6.

Figure 6

Effect of DJ‐1 deletion on mitochondrial oxidative stress. A and B, mRNA expression the markers of mitophagy; Bcl2 interacting protein 3 (Bnip3), Bcl2 interacting protein 3 like (Bnip3L), PTEN‐induced putative kinase 1 (Pink1), the markers of mitochondrial fusion and fission; fission mitochondrial 1 (Fis1), mitofusin 1 (Mfn 1), mitofusin 2 (Mfn 2), mitochondrial biogenesis; peroxisome proliferator‐activated receptor γ coactivator‐related 1 (Pprc1), peroxisome proliferator‐activated receptor γ coactivator 1 α (Pgc1‐α), and nuclear respiratory factor 1 (Nrf‐1) and mitochondrial heat shock proteins 40 and 60 (mtHsp40 and mtHsp60) as well as uncoupling proteins 2 (Ucp2) were determined in kidneys of DJ‐1 −/− mice and wild‐type littermates by quantitative real‐time polymerase chain reaction and normalized by GAPDH. C, mtHsp60 protein expression was assessed by Western blot and normalized by GAPDH protein expression. Data are expressed as mean±SEM, n=4/group. *P<0.05 vs wild‐type mice; # P<0.05 vs DJ1 −/− mice treated with vehicle, 2‐way ANOVA.