Table 1.
Sequences for Mouse Primers Used for Real‐Time Polymerase Chain Reaction of the Following Genes: Bnip3, Bnip3l, Pink1, Pprc1, Nrf‐1Pgc1‐a, Fis1, Mfn 1, Mfn 2, UCPs, Hsp, CHOP, GRP94, sXBP‐1, ATF‐4, and ATF‐6
| Gene | Mouse Sequence |
|---|---|
| Nix/BNIP3L | F: CCT CGT CTT CCA TCC ACA AT20 |
| R: GTC CCT GCT GGT ATG CAT CT | |
| Bnip3 | F: GCT CCC AGA CAC CAC AAG AT20 |
| R: TGA GAG TAG CTG TGC GCT TC | |
| PINK1 | F: GCT GAT CGA GGA GAA GCA G21 |
| R: GAT AAT CCT CCA GAC GGA AGC | |
| PPRC1 | F: TGC CTT GCA GTT ACT CAT GC22 |
| R: CTG ACT TGC ACT GGC AGG TA | |
| Nrf1 | F: TGG TCC AGA GAG TGC TTG TG22 |
| R: TTC CTG GGA AGG GAG AAG AT | |
| PGC1‐a | F: CCG TAA ATC TGC GGG ATG ATG23 |
| R: CAG TTT CGT TCG ACC TGC GTA A | |
| Fis1 | F: AGG CCG TGC TGA ACG AGC TG24 |
| R: GGT AGT TGC CCA CGG CCA GG | |
| Mfn1 | F: TGG TCA CAC AAC CAA CTG CT25 |
| R: ACC AAT GCC TTT GCA AGT TGT | |
| Mfn2 | F: GGG GCC TAC ATC CAA GAG A25 |
| R: AAA AAG CCA CCT TCA TGT GC | |
| Ucp‐1 | F: TAT CAT CAC CTT CCC GCT G26 |
| R: GTC ATA TGT TAC CAG CTC TG | |
| Ucp‐2 | F: TCT ACA ATG GGC TGG TCG C26 |
| R: CAA GCG GAG AAA GGA AGG C | |
| mtHsp40 | F: GCC TGT ATG AGA CAA TCA ATG TGA CGA23 |
| R: GTG AAT GTA GTG GTC ACC ATA GCC A | |
| mtHSP70 | F: CGT GAG CAA CAG ATT GTA ATC CAG T26 |
| R: GCC ATA TTA ACT GCT TCA ACA CGT TC | |
| mtHsp60 | F: TCA TCG GAA GCC ATT GGT CAT A24 |
| R: GCT TTG ACT GCC ACA ACC TGA A | |
| CHOP | F: ATA TCT CAT CCC CAG GAA ACG27 |
| R: TCT TCC TTG CTC TTC CTC CTC | |
| Caspase 12 | F: ATG CTG ACA GCT CCT CAT GGA28 |
| R: TGA GAG CCA GAC GTG TTC GT | |
| GRP‐94 | F: TGG GTC AAG CAG AAA GGA G29 |
| R: TCT CTG TTG CTT CCC GAC TT | |
| sXBP‐1 | F: GAG TCC GCA GCA GGT G30 |
| R: GTG TCA GAG TCC ATG GGA | |
| ATF‐4 | F: GCA AGG AGG ATG CCT TTT C31 |
| R: GTT TCC AGG TCA TCC ATT CG | |
| ATF‐6 | F: TGG GAG TGA GCT GCA AGT GT29 |
| R: ATA AGG GGG AAC CGA GGA G |
Bcl2 Interacting Protein 3 (Bnip3), Bcl2 Interacting Protein 3 Like (Bnip3l), PTEN‐Induced Putative Kinase 1 (Pink1), Peroxisome Proliferator‐Activated Receptor Gamma Coactivator‐Related 1 (Pprc1), Nuclear Respiratory Factor 1 (Nrf‐1), Peroxisome Proliferator‐Activated Receptor Gamma Coactivator 1 Alpha (Pgc1‐a), Fission Mitochondrial 1 (Fis1), Mitofusin 1 (Mfn 1), Mitofusin 2 (Mfn 2), Uncoupling Proteins (UCPs), Mitochondrial Heat Shock Protein (Hsp), C/EBP‐Homologous Protein (CHOP), Caspase 12, Glucose‐Regulated Protein (GRP94), Activating Splice X Box‐Binding Protein 1 (sXBP‐1), Transcription Factor 4 (ATF‐4), and Activating Transcription Factor 6 (ATF‐6).