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. 2019 Apr 25;8(9):e010662. doi: 10.1161/JAHA.118.010662

Figure 2.

Figure 2

Leucine‐induced activation of mTORC1 promote pro‐oxidant environment in endothelial cells. Mouse lung endothelial cells were stimulated with 10 mmol/L leucine for 16 hours under serum‐free conditions. A, Representative Western blot images and quantification data (expressed as arbitrary units) of mouse lung endothelial cells for indicated proteins (n=6 independent experiments). mRNA levels of indicated (B) Reactive oxygen species degrading enzymes, (C) NADPH oxidases, and (D) inflammatory markers (n=6 independent experiments). CAT indicates catalase; IL, interleukin; mTORC1, mechanistic target of rapamycin complex 1; NOX, NADPH oxidase; SOD, superoxide dismutase. *P<0.05 leucine vs control.