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. 2019 Apr 25;8(9):e010662. doi: 10.1161/JAHA.118.010662

Figure 4.

Figure 4

Impairment of endothelial‐mediated relaxation elicited by mTORC1 activation involves oxidative stress. A, Representative Western blot images and quantification data (expressed as arbitrary units) of aortic rings infected (24 hours) with Ad‐GFP or Ad‐S6KCA for indicated proteins (n=6–9/group). Vascular reactivity responses of adenoviral infected aortic rings to (B) endothelial‐dependent acetylcholine, (C) endothelial‐independent sodium nitroprusside, and contractile responses to (D) prostaglandin F2α (n=8/group). A subset of aortic rings was pre‐incubated with Tempol (100 μmol/L; 30 minutes) before cumulative response curves. ACh indicates acetylcholine; Ad‐S6KCA, adenoviral S6‐kinase constitutively active; GFP, green fluorescent protein; mTORC1, mechanistic target of rapamycin complex 1; PGF2α, prostaglandin F2α; SNP, sodium nitroprusside. *P<0.05 vs Ad‐GFP; †P<0.05 vs Ad‐S6KCA+Tempol; ‡P<0.05 Ad‐GFP vs Ad‐S6KCA+Tempol.