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. 2019 Apr 29;8(9):e010917. doi: 10.1161/JAHA.118.010917

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© 2019 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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mRNA expression levels of RGS2 and RGS5 during pregnancy in wild‐type (WT) and knockout mouse uterine arteries, mesenteric arteries, and aorta. mRNA expression was determined from isolated uterine arteries, mesenteric arteries, and aorta by RT‐qPCR. A, mRNA expression of RGS2, RGS4, and RGS5 in uterine arteries from WT mice at nonpregnant (NP), gestation day 10 (D10), gestation day 15 (D15), gestation day 18 (D18), and postpartum day 3 (PPD3; RGS2: NP n=3, D10 n=5, D15 n=6, D18 n=3, and PPD3 n=5; RGS4: NP n=4, D10 n=4, D15 n=4, D18 n=4, and PPD3 n=3; RGS5: NP n=7, D10 n=4, D15 n=9, D18 n=5, and PPD3 n=4). B, mRNA expression of RGS2, RGS4, and RGS5 in uterine arteries from Rgs2^−/− mice at NP, D10, D15, D18, and PPD3 (RGS4: NP n=4, D10 n=5, D15 n=4, D18 n=5, and PPD3 n=5; RGS5: NP n=6, D10 n=5, D15 n=4, D18 n=5, and PPD3 n=5). C, mRNA expression of RGS2, RGS4, and RGS5 in uterine arteries from Rgs5^−/− mice at NP, D10, D15, D18, and PPD3 (RGS2: NP n=6, D10 n=3, D15 n=5, D18 n=5, and PPD3 n=10; RGS4: NP n=5, D10 n=3, D15 n=5, D18 n=5, and PPD3 n=5). D, mRNA expression of RGS2, RGS4, and RGS5 in mesenteric arteries from WT mice at NP, D15, and PPD3 (RGS2: NP n=3, D15 n=3, and PPD3 n=4; RGS4: NP n=4, D15 n=3, and PPD3 n=4; RGS5: n=4, D15 n=3, and PPD3 n=4). E, mRNA expression of RGS2, RGS4, and RGS5 in mesenteric arteries from Rgs2^−/− mice at NP, D15, and PPD3 (RGS4: NP n=3, D15 n=3, and PPD3 n=3; RGS5: NP n=3, D15 n=3, and PPD3 n=3). F, mRNA expression of RGS2, RGS4, and RGS5 in mesenteric arteries from Rgs5^−/− mice at NP, D15, and PPD3 (RGS2: NP n=3, D15 n=3, and PPD3 n=3; RGS4: NP n=3, D15 n=3, and PPD3 n=3). G, mRNA expression of RGS2, RGS4, and RGS5 in aorta from WT mice at NP, D15, and PPD3 (RGS2: NP n=3, D15 n=3, and PPD3 n=3; RGS4: NP n=3, D15 n=3, and PPD3 n=3; RGS5: NP n=3, D15 n=3, and PPD3 n=3). H, mRNA expression of RGS2, RGS4, and RGS5 in aorta from Rgs2^−/− mice at NP, D15, and PPD3 (RGS4: NP n=3, D15 n=3, and PPD3 n=3; RGS5: NP n=3, D15 n=3, and PPD3 n=3). I, mRNA expression of RGS2, RGS4, and RGS5 in aorta from Rgs5^−/− mice at NP, D15, and PPD3 (RGS2: NP n=3, D15 n=3, and PPD3 n=3; RGS4: NP n=3, D15 n=3, and PPD3 n=3). Values are mean±SE. *^,**P<0.05, 0.01 vs NP.