Figure 6.4.

Effect of EHop-016 on cell viability and Rac activity. MDA-MB-435 cells were treated with vehicle (0.1% DMSO), or varying concentrations of EHop-016 (0–10 μM) for 24 h. Cell viability was measured using the MTT cell survival and proliferation kit (Millipore, Inc). Rac activity was measured from cell lysates by a G-LISA Rac1 Activation Assay (Cytoskeleton, Inc., Denver, CO). The mean values SEM (N = 3) are presented relative to vehicle (100%). All data points for Rac activity > 0.5 μM EHop-016, and for viability at >5.0 μM EHop-016, were statistically significant compared to vehicle controls (p≤0.05).