Figure 5.

Adhesion to CII results in a reduced phosphorylation of focal adhesion kinase (FAK) at Y397 in ADAM15 expressing cells. Cells were adhered to CII and lysed after 15, 30 and 60 min. Cell lysates (40 μg) were applied to SDS/PAGE and immunoblotted using anti‐pY397 FAK antibodies. (A) ADAM15 (+)‐transfected cells exhibit a 2–3 times reduced phosphorylation of FAK compared with vector control (−) cells, whereas (B) ADAM15 Δ cyto expressing cells (ΔC) show an identical activation of FAK during adhesion. As loading control, all filters were stripped and reprobed with anti‐FAK antibodies and expression of ADAM15 and ADAM15 Δ cyto was controlled using anti‐ADAM15 antibodies. Optical density of the bands was analysed using ImageJ software and normalized to non‐phosphorylated FAK expression.