Figure 1.

Effects of platelet lysate (PL) exposure on scratch wound healing of confluent fibroblasts. Cells cultured in 12‐well plates were mechanically scratched with a sterile 0.1–10‐μl pipet tip, and then maintained for 6 or 24 hrs at 37°C in the presence or absence of 20% PL. Upper panel. Phase contrast micrographs of scratched fibroblasts that were fixed and stained with blue‐toluidine 24 hrs after scratching. Scale bar: 300 μm. Lower panel. Each bar indicates mean ± S.D. (n= 8) of wound closure rates expressed as the difference between wound width at 0 hrs and at 6 or 24 hrs. The mean of controls at 6 hrs was set at 100%. All pairwise comparisons yield significant differences according to the Tukey’s test (P < 0.01).