Figure 2.

ERK inhibition exerts anti‐angiogenic effects. (A) HMECs were either kept untreated (control) or stimulated with U0126 (10 μM). (B) HUVEC monolayers were wounded and stimulated with ECGM alone or a medium containing U0126 (10 μM) for 16 hrs. *P < 0.05 versus control, Kruskal–Wallis one‐way ANOVA on ranks (Dunn's method). (C) HMECs were incubated with U0126 (10 μM). After 16 hrs, tube formation was observed using the TILLvisON system. (D) One representative image of the chicken CAM is shown. Fertilised eggs were stimulated either with FGF (1ng/dish) alone or with FGF and U0126.