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. 2008 Jul 9;13(8b):2083–2108. doi: 10.1111/j.1582-4934.2008.00415.x

Figure 5.

Figure 5

qRT‐PCR analysis of selected chemokines in endothelial cells stimulated by immobilized or soluble FGF2. Microvascular 1G11 cells were incubated for 24 hrs on FGF2‐coated dishes (immobilized FGF2) or stimulated for 24 hrs with 30 ng/ml of FGF2 (soluble FGF2) onto non‐coated dishes. Total RNA was reverse transcribed to cDNA and analysed by real‐time PCR. Data are presented as the expression ratio of each target gene relative to an untreated control. Expression levels were normalized to β‐actin gene. The bars show mean values ± SD (n= 3).