Figure 6.
The conditioned medium (CM) from FGF2‐stimulated microvascular endothelial cells is chemotactic to monocytes. (A) The migration of human monocytes towards different dilutions of the CM from non‐stimulated (Control_CM) and FGF2‐stimulated (FGF2_CM) 1G11 endothelial cells was quantified as described in ‘Materials and methods’. Data are expressed as the mean number of migrated cells per field. (B) Inhibition of the chemotactic response of 1G11 endothelial cells towards FGF2‐CM by different concentrations of the pan‐chemokine inhibitor M3. Chemotaxis was tested at the dose of FGF2_CM that induced the maximal chemotactic responses (1/300 dilution) and compared to the equivalent dilution of Control_CM. Data are expressed as the number of migrated cells per field.