Table 4.
FGF2‐upregulated genes in murine microvascular 1G11 endothelial cells related to angiogenesis, bone formation, and inflammation
| Growth factors, cytokines and chemokines | Symbol | Biological Process | Fold change |
|---|---|---|---|
| Chemokine (C‐C motif) ligand 7 | Ccl7 | I | 3.1 |
| Chemokine (C‐X3‐C motif) ligand 1 | Cx3cl1 | A/I | 2.0 |
| Chemokine (C‐X‐C motif) ligand 1 | Cxcl1 | A/I | 2.0 |
| Chemokine (C‐X‐C motif) ligand 16 | Cxcl16 | A/I | 2.0 |
| Connective tissue growth factor | Ctgf | A/B | 2.3 |
| Ephrin B2 | Efnb2 | A/B | 2.1 |
| Fibroblast growth factor 2 | Fgf2 | A/B/I | 2.3 |
| Gremlin 1 | Grem1 | A | 3.1 |
| Heparin‐binding EGF‐like growth factor | Hbegf | A | 8.4 |
| Interleukin 6 | Il6 | A/B/I | 3.8 |
| Leukemia inhibitory factor | Lif | B/I | 2.3 |
| Platelet‐derived growth factor, B polypeptide | Pdgfb | A | 3.2 |
| Prolactin family 2, subfamily c, member 2/Proliferin | Prl2c2/Pfl | A | 13.5 |
| Secreted phosphoprotein 1/osteopontin | Spp1/Opn | A/B/I | 12.2 |
| Slit homolog 2 (Drosophila) | Slit2 | A/I | 3.0 |
| Thrombospondin 1 | Thbs1 | A/B/I | 2.7 |
| Transforming growth factorα | Tgfa | A | 2.2 |
| Transforming growth factor, β1 | Tgfb1 | A/B/I | 2.2 |
| Tumor necrosis factor, α‐induced protein 2 | Tnfaip2 | A | 2.3 |
| Membrane receptors and adhesion molecules | Symbol | Biological Process | Fold change |
|---|---|---|---|
| Anthrax toxin receptor 2 | Antxr2 | A | 2.7 |
| Calcitonin receptor‐like | Calcrl | A/B | 2.3 |
| CD44 antigen | Cd44 | A/I | 6.1 |
| Coagulation factor III | F3/Tf | A/I | 2.9 |
| Endothelial differentiation sphingolipid G‐protein‐coupled receptor 1 | Edg1 | A | 4.1 |
| Integrin α5 (fibronectin receptor α) | Itga5 | A | 2.0 |
| Interleukin 1 receptor accessory protein | Il1rap | I | 2.3 |
| Junction adhesion molecule 2 | Jam2 | I | 2.3 |
| Lymphatic vessel endothelial hyaluronan receptor 1 | Lyve1 | A | 2.4 |
| Oncostatin M receptor | Osmr | A/B/I | 2.2 |
| Plasminogen activator, urokinase receptor | Plaur | A/B/I | 3.2 |
| Prostaglandin E receptor 4 (subtype EP4) | Ptger4 | A/B/I | 4.9 |
| Tumor necrosis factor receptor superfamily, member 12a | Tnfrsf12a | A/B/I | 2.9 |
| Vascular cell adhesion molecule 1 | Vcam1 | I | 3.1 |
| Transcriptional regulators | Symbol | Biological Process | Fold change |
|---|---|---|---|
| Early growth response 1 | Egr1 | A | 3.4 |
| Early growth response 2 | Egr2 | B | 5.6 |
| Runt‐related transcription factor 1 | Runx1 | A/B | 2.8 |
| Runt‐related transcription factor 2 | Runx2 | A/B | 2.1 |
| Ankyrin repeat domain 1 (cardiac muscle) | Ankrd1 | A | 3.6 |
| Others | Symbol | Biological Process | Fold change |
|---|---|---|---|
| Matrix Gla protein | Mgp | B | 4.1 |
| Matrix metallopeptidase 13 | Mmp13 | A/B | 22.1 |
| Prostaglandin‐endoperoxide synthase 2 | Ptgs2/Cox‐2 | A/B/I | 18.5 |
| Serine (or cysteine) peptidase inhibitor, clade E, member 1 | Serpine1 | A/I | 3.0 |
| Serine (or cysteine) peptidase inhibitor, clade B, member 2 | Serpinb2 | A | 5.6 |
| Tissue inhibitor of metalloproteinase 1 | Timp1 | A/I | 2.5 |
Selected FGF2‐upregulated genes (fold change > 2, P < 0.05, see Table 2) were classified in terms of their association with angiogenesis (A), bone formation (B) and inflammation (I) processes, according to the GO database and bibliographic searching.