Table 5.

Immunoistochemical characterization of the inflammatory infiltrate in FGF2‐Matrigel plugs

Treatment	CD31+	CD45+	CD11b+	F4/80+	GR‐1+	CD4+	CD8+
PBS	1074 ± 748	7682 ± 3220	7002 ± 601	2316 ± 186	61 ± 63	0	0
FGF2	12,228 ± 2024	37,467 ± 5900	13,469 ± 2372	17,401 ± 7187	82 ± 115	154 ± 169	654 ± 834

Eight μm frozen sections of Matrigel plugs containing PBS or FGF2 (five plugs per group) were immunostained for the indicated antigens. Then, the corresponding immunoreactive areas were analysed in five microscopic fields per Matrigel section (two sections per Matrigel plug) using the ImagePro Plus software. Analysis was performed on plugs on day 7 after implantation. Data (mean ± SD) are expressed as μm² of immunoreactive area per microscopic field (0.38 mm²).