Figure 3.

Modulation of skin GvH type alloreactivity by USSC. Irradiated unstimulated (A) or IFNγ+TNFα pre‐stimulated USSC (C) were co‐cultured with an allogeneic MLR using irradiated PBMC of the skin donor as stimulator cells and PBMC of an unrelated, HLA‐mismatched donor as responders (responder‐to‐USSC ratio = 10:1). MLR‐primed responder cells were added to skin biopsies and co‐cultured for an additional 3 days before histopathological evaluation. Skin explants co‐cultured with responder cells primed in the absence of USSC or with medium alone served as positive and negative controls, respectively (B and D).