Figure 1.

Putative E2F1‐binding sites are located in Siah1 promoter (A) Human genomic sequence of Siah1 gene from –345 to +174 is shown. Shaded part represents the first exon of Siah1. The boxed are the predicted E2F1‐binding sites. The arrow under the DNA sequence represents the start/end position of some indicated promoters. (B) Alignment of the putative E2F‐binding sites with the consensus sequence. (C) Schematic representation of various Siah1 promoter deletion constructs. Putative E2F‐binding sites are indicated. The constructs harbouring different fragment of Siah1 promoter were cloned into pGL3‐Basic reporter vector (Promega Corporation).