Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
E2F1 binds to Siah promoter in vivo. H1299 cells were transfected with or without pcDNA‐HA‐E2F1 expression vector. Twenty‐four hours after transfection, cells were harvested and subjected to ChIP assay. ChIP products were amplified by PCR reaction. β‐actin was used as a negative control. M: DNA marker; Mock: control IP without nuclear lysate; No Ab: IP without E2F1 antibody; E2F1 Ab: IP with E2F1 antibody. For the right four lanes, cells were expressing with pcDNA3‐E2F1.
