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. 2008 Oct 13;13(8b):2039–2052. doi: 10.1111/j.1582-4934.2008.00549.x

Figure 2.

Figure 2

Regulation of the steady‐state level of survivin gene transcript. (A) BCR/ABL does not affect survivin mRNA stability. 32D‐BCR/ABL and 32Dcl3 cells were incubated with 5 μg/ml actinomycin D and then harvested at indicated time. RNA was extracted and RT‐PCR analysis of survivin and vegf mRNA levels was performed. The amount of mRNA at each time‐point was normalized to that of the initial stages. The half‐time was calculated by drawing the best‐fit linear curve with mRNA versus time. One representative result from two independent experiments is shown. (B) Inhibition of BCR/ABL diminishes survivin promoter activity. K562 cells were transfected with indicated promoter reporter vectors and cultured for 8 hrs, after which cells were incubated for 16 hrs in the absence or presence of imatinib. The promoter activity was measured by normalizing firefly to renilla luciferase activity. Mean values ± S.D. from three independent experiments in triplicate are shown. *P≤ 0.001, #no significance as compared to untreated group.