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. 2008 Jul 24;13(8b):2534–2546. doi: 10.1111/j.1582-4934.2008.00429.x

Figure 5.

Figure 5

Figure 5

Figure 5

IL‐8 blockade inhibits SFLLRN‐induced migration of early EPC but not their proliferation and differentiation. (A) The effect of SFLLRN 75 μM on CB late EPC proliferation was evaluated by measuring pNPP release at 405 nm in EBM2 medium (mean ± S.E.M.). An IL‐8 blocking antibody had no effect on cell proliferation. *P < 0.05. (B) Quantitative analysis of network length. EPC were stimulated with SFLLRN 75 μM for 4 hrs before being used in the tubule formation assay on Matrigel for 18 hrs, in the presence or absence of a polyclonal antibody against human IL‐8. IL‐8 blockade did not inhibit SFLLRN‐induced pseudotube formation. *P < 0.05. (C) Early‐EPC migration towards conditioned medium (CM) of late EPC activated with SFLLRN for 48 hrs. The control is represented by early‐EPC migration toward CM of untreated late EPC (100% migration). CM of late EPC after PAR‐1 activation enhanced the migration of early EPC. This effect was abrogated by a polyclonal antibody against human IL‐8. The mean and S.E.M. of three experiments are shown. *P < 0.05.