Table 3.

Effect of PAR‐1 activation in CB late EPC on mRNA expression of IL‐8 and other genes activated by AP‐1 and/or NF‐κB pathways

	Target	1 hr	4 hrs
IL‐8	AP‐1/ NF‐κB	53.1 ± 32*	30.1 ± 25*
MMP1	AP‐1	1.9 ± 0.6*	2 ± 0.6*
ATF3	AP‐1	5.6 ± 1.6*	1.5 ± 0.7
Jun‐c	AP‐1	1.1 ± 0.1	1 ± 0.4
Jun‐b	AP‐1	104.4 ± 130*	19.2 ± 14
Jun‐d	AP‐1	1.6 ± 0.6	1.1 ± 0.4
Fos	AP‐1	1.6 ± 1	0.3 ± 0.2*
Fos‐b	AP‐1	46 ± 44*	0.9 ± 0.6
GADD45B	NF‐κB	6.2 ± 3*	1.7 ± 0.4*
BCL2A1	NF‐κB	59.2 ± 56*	50.1 ± 46*
IER3S	NF‐κB	0.9 ± 0.5	0.5 ± 0.1*
NFKB‐1	NF‐κB	1.9 ± 0.1*	2.1 ± 0.6*
NFKB‐2	NF‐κB	2.9 ± 1*	5.9 ± 4*
REL	NF‐κB	1.5 ± 0.6	0.4 ± 0.1*
REL‐A	NF‐κB	1.3 ± 0.6	1 ± 0.3
REL‐B	NF‐κB	5.2 ± 2*	6.1 ± 3*

EPC were stimulated with SFLLRN for 1 or 4 hrs after 16 hrs of serum and growth‐factor privation. MRNAs were measured by real‐time quantitative RT‐PCR and normalized to TBP mRNA (mean ± S.E.M., n= 3). *P < 0.05, compared with unstimulated cells (= 1).