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. 2019 Apr 26;10:379. doi: 10.3389/fgene.2019.00379

Table 2.

Frequencies of mutations induced by VQRn-pBE at target sites on the OsWaxy gene with NGAT and NGAC PAMs in rice resistant calli and T0 plants.

Target site PAM Calli
T0 plants
No. of C-to-T mutants No. of transgenic calli C-to-T frequency in calli (%) No. of C-to-T mutants No. of transgenic T0 plants C-to-T frequency in T0 plants (%)
W-T11 NGAT 2 15 13.3 1 25 4
W-T12 2 15 13.3 1 25 4
W-T13 0 15 0 1 17 5.9
W-T14 0 15 0 0 14 0
W-T15 NGAC 10 15 66.7 7 17 41.2
W-T16 0 15 0 0 17 0
W-T17 0 15 0 0 17 0
W-T18 0 15 0 0 16 0