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In vitro transcription components for mRNA generation.
| T7 RNA Polymerase In Vitro Transcription Reactions | ||||
|---|---|---|---|---|
| Component | Stock (mM) | Final Concentration (mM) | Quantity (μL) 1× Reaction | Quantity (μL) 50× Reactions |
| 10× RNAPol Reaction Buffer | 1.00 | 50 | ||
| ATP | 100 | 0.5 | 0.10 | 5 |
| GTP | 100 | 0.5 | 0.10 | 5 |
| CTP | 100 | 0.5 | 0.10 | 5 |
| UTP | 100 | 0.5 | 0.10 | 5 |
| DNA Template (ng/μL)* | 1000 | 500 | 0.50 | 25 |
| T7 RNA Polymerase | 200 | 2.6 | 2.00 | 100 |
| Rnase Inhibitor, Murine | 200 | 3.3 | 0.50 | 25 |
| Sterile Deionized Water | 15.60 | 780 | ||
| Reaction Volume (μL): | 20 | |||
