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. Author manuscript; available in PMC: 2019 May 13.
Published in final edited form as: Nat Protoc. 2018 Nov;13(11):2615–2642. doi: 10.1038/s41596-018-0055-0

Figure 4 |. Expected results of CIRCLE-seq experiments.

Figure 4 |

(a, b) Visualization of detected off-targets identified by CIRCLE-seq aligned against intended target site for Cas9:sgRNA complexes targeted against EMX1 and VEGFA genes, respectively. The intended target sequence is shown in the top line and off-target sites are ordered top to bottom by CIRCLE-seq read count. Mismatches to the intended target sequence are indicated by colored nucleotides, and read counts shown at the end of each line. The on-target site is marked with a star. For space considerations, this figure shows a listing of only the top CIRCLE-seq off-target sites detected in this experiment. (c, d) Manhattan plots of CIRCLE-seq detected off-target sites, with bar heights representing read count and organized by chromosomal position for EMX1 and VEGFA site 1, respectively.