| Component | Amount (ml) | 10× concentration |
|---|---|---|
| Tris-HCl, pH 8.0, 1M | 5 | 100 mM |
| NaCl 5M | 5 | 500 mM |
| EDTA 0.5M | 1 | 10 mM |
| H2O | 39 | |
| Total | 50 | |
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