Component | Volume (μl) | Final concentration |
---|---|---|
Exonuclease I Reaction Buffer (10×) | 5 | 1× |
Lambda Exonuclease (5 U/μl) | 4 | 0.4 U/μl |
Exonuclease I (E. coli) (20 U/μl) | 1 | 0.4 U/μl |
Total Lambda Exo/ExoI master-mix | 10 |
Component | Volume (μl) | Final concentration |
---|---|---|
Exonuclease I Reaction Buffer (10×) | 5 | 1× |
Lambda Exonuclease (5 U/μl) | 4 | 0.4 U/μl |
Exonuclease I (E. coli) (20 U/μl) | 1 | 0.4 U/μl |
Total Lambda Exo/ExoI master-mix | 10 |