| Component | Volume (μl) | Final concentration |
|---|---|---|
| Nuclease-free water | 8 | |
| T4 DNA Ligase Buffer (10×) | 10 | 1× |
| T4 DNA Ligase (400 U/μl) | 2 | 8 U/μl |
| Total Circularization master-mix | 20 | |
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