| Component | Volume (μl) | Final concentration |
|---|---|---|
| Cas9 Nuclease Reaction Buffer (10×) | 5 | 1× |
| Cas9 Nuclease, S. pyogenes (1 μM) | 4.5 | 90 nM |
| In vitro transcribed sgRNA (3 μM) | 1.5 | 90 nM |
| Total cleavage master-mix | 11 | |
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