| Component | Volume (μl) | Final concentration |
|---|---|---|
| sgRNA Top 100 μM | 10 | 10 μM |
| sgRNA Bottom 100 μM | 10 | 10 μM |
| STE 10× | 10 | 1× |
| Nuclease-free H2O | 70 | |
| Total | 100 | |
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