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. Author manuscript; available in PMC: 2019 May 13.
Published in final edited form as: Nat Protoc. 2018 Nov;13(11):2615–2642. doi: 10.1038/s41596-018-0055-0
Component 1 reaction (μl) Final Concentration
2× ddPCR Supermix for probes 11 1×
Probe oSQT1310 (100 μM) (Table 2) 0.055 250 nM
Probe oSQT1311 (100 μM) (Table 2) 0.055 250 nM
Primer oSQT1274 (100 μM) (Table 2) 0.099 450 nM
Primer oSQT1275 (100 μM) (Table 2) 0.099 450 nM
Nuclease-free water 6.292
Total ddPCR mix 17.6