| Component | Volume (μl) | Final concentration |
|---|---|---|
| CutSmart buffer (10×) | 5 | 1× |
| sgRNA plasmid (3-5 μg) | Variable | variable |
| HindIII-HF (20 U/μl) | 4 | 1.6 U/μl |
| Nuclease-free H2O | to 50 | |
| Total | 50 | |
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