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. Author manuscript; available in PMC: 2019 May 13.
Published in final edited form as: Nat Protoc. 2018 Nov;13(11):2615–2642. doi: 10.1038/s41596-018-0055-0
Component Volume (μl) Final concentration
T7 10× Reaction Buffer 2 1×
HindIII Digested DNA 8 variable
dATP (75 mM) 2 7.5 mM
dUTP (75 mM) 2 7.5 mM
dCTP (75 mM) 2 7.5 mM
dGTP (75 mM) 2 7.5 mM
Enzyme Mix 2
Total 20