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. Author manuscript; available in PMC: 2019 May 13.
Published in final edited form as: Nat Protoc. 2018 Nov;13(11):2615–2642. doi: 10.1038/s41596-018-0055-0
Component Volume (μl) Final concentration
Phusion Hot Start Flex 2× Master Mix 25 1×
oSQT1143 F 10 μM (Table 2) 0.5 0.1 μM
oSQT1144 R 10 μM (Table 2) 0.5 0.1 μM
gDNA (U2OS gDNA, 100 ng, 20 ng/μl, from Step 8) 5 2 ng/μl
H2O 19
Total 50