Figure 4–

Typical trapping behavior and data in a V1 microchannel at the 27 µm gate (top left illustration, the light blue box represents the area in the images). The data depicted is of Listeria monocytogenes 1/2a with an applied potential of 350 V (85 V/cm). Negative dielectrophoresis results in the collection of the material in a crescent-shaped band on the inlet side of the gate (bottom left). Trapping occurs at applied potentials above a threshold value specific to the serovar. The bacteria are continuously collected when potential is applied. Three time points are shown as images, color coded corresponding to lines on FI vs t plot demonstrating the channel before, during, and after trapping (red = 0 s, green = 10 s, black = 28 s; n = 12, ROI described in the text). The grey region around each data point in plot is the 95% confidence interval. Inset is typical data for a single trial. The reversible nature of capture of the bacteria is demonstrated in the last image, at 28 s, as the bacteria disperse back into the microchannel immediately after the voltage is removed.