Table 4.
Kinetic parameters of α-amylase-catalyzed degradation of starch in the absence and presence of Luffa cylindrica seed protein hydrolysates.
| Catalytic Parameter | No Inhibitor | PepH (mg/mL) |
TrypH (mg/mL) |
AlcH (mg/mL) |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0.50 | 1.00 | 1.50 | 0.50 | 1.00 | 1.50 | 0.50 | 1.00 | 1.50 | ||
| Km (mg/mL) | 1.75 | 1.57 | 1.67 | 1.60 | 1.67 | 1.83 | 1.50 | 1.67 | 1.57 | 1.67 |
| Vmax | 2500 | 1429 | 1667 | 2000 | 1250 | 1667 | 1667 | 1667 | 1429 | 1111 |
| CE (μmol/mL/min) | 1429 | 910 | 998 | 1250 | 749 | 911 | 1111 | 998 | 910 | 665 |
| Ki | 4.21 | 3.57 | 1.46 | |||||||
Km – Michaelis constant in the presence and absence of hydrolysate; Vmax – Maximum reaction rate in the presence and absence of hydrolysate; CE – Catalytic efficiency; Ki – Enzyme-inhibitor dissociation constant; PepH, TrypH and AlcH – Peptic, tryptic and Alcalase hydrolysates respectively.