Table 5.
Kinetic parameters of α-glucosidase-catalyzed degradation of starch in the absence and presence of Luffa cylindrica seed protein hydrolysates.
| Catalytic Parameter | No Inhibitor |
AlcH (mg/mL) |
TrypH (mg/mL) |
PepH (mg/mL) |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0.50 | 1.00 | 1.50 | 0.50 | 1.00 | 1.50 | 0.50 | 1.00 | 1.50 | ||
| Km (mg/mL) | 6.31 | 1.27 | 1.82 | 3.31 | 0.99 | 1.2 | 2.33 | 1.2 | 1.38 | 2.12 |
| Vmax(x10−2) | 0.33 | 0.05 | 0.07 | 0.15 | 0.05 | 0.07 | 0.12 | 0.61 | 0.07 | 0.12 |
| CE (μmol/mL/min x 10−4) | 5.20 | 3.90 | 3.80 | 4.50 | 5.00 | 5.80 | 5.20 | 5.00 | 5.00 | 5.70 |
| Ki. | 8.41 | 49.83 | 10.51 | |||||||
Km – Michaelis constant in the presence and absence of hydrolysate; Vmax – Maximum reaction rate in the presence and absence of hydrolysate; CE – Catalytic efficiency; Ki – Enzyme-inhibitor dissociation constant; PepH, TrypH and AlcH – Peptic, tryptic and Alcalase hydrolysates respectively.