Figure 6.

Impact of using HPRT as a normalization standard on gene expression. Normal and malignant HPRT levels were used as a normalization to compare the expression of the remaining 9 endogenous control genes. Total gene expression was plotted with either “normal” or “malignant” HPRT levels within each cancer type as the normal standard to which the fold changes in all other proteins was determined. We found that when using either normal or malignant HPRT levels there was significant variability in the other endogenous control gene expression profiles.