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. 2019 Feb 26;11(3):516–531. doi: 10.1080/19420862.2019.1571879

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 5. — Frequency of eighteen neutral IGHD fragments before and after filtration as determined by NGS. Top panel: Frequency of PLs and FLs. The first two bars per IGHD correspond to the PLs (PL1: blue; PL2: dark red). The other two bars per IGHD correspond to the FLs (FL1: light blue; PL2: orange). A horizontal bold line at 6% represents the expected frequency of an even distribution of the 18 IGHD fragments. Two black lines at 4% and 8% represents the range of the frequency variation in the PLs. Bottom panel: Difference in frequency (ΔF) of FLs with respect to PLs. ΔF was calculated as: IGHD frequency of the FL minus IGHD frequency of PL and normalized by IGHD frequency of PL x 100; that is: [(IGHD of FL – IGHD of PL)/IGHD of PL] x100.