Figure 2.

Root tip angle of NR mutants after gravistimulation.

Wild-type, nia1, nia2, and nia1/nia2 A. thaliana seedlings were grown vertically on 0.5x Murashige and Skoog salt (MS), 0.8% agar and 1% sucrose for 4 days. Seedlings were transferred to fresh MS medium and plates were mounted in front of a photo scanner (Epson Perfection V600) and let set for 60 min before 90-degree rotation. For each time series of individual seedlings, root tip angle was measured on digital images using FIJI.¹⁷ Means (±SE) are shown. P values in comparison to wt were calculated with two-tailed Mann–Whitney test, ***p < 0.0001. The average values of at least four independent experiments were analyzed, n ≥ 20.