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. 2019 Feb 6;11(3):463–476. doi: 10.1080/19420862.2019.1565748

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Figure 4. — Structural changes revealed from DEPC CL-MS experiments are validated using rituximab activity assays. The structural integrity of the F_c region is evaluated by (a) a rituximab bridging ELISA that measures F_c binding to a capture antibody and (b) an Alamar blue assay that measures complement dependent cytotoxicity (CDC). The structural integrity of the F_ab region is assessed by (c) a Raji cell pull-down assay that measures F_ab binding to CD20 antigen on B cells. For each temperature condition, the means of two groups i.e. stressed vs. control (37°C) were compared using student t-test at 95% confidence level. Each assay was performed in triplicate (n = 3).