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. Author manuscript; available in PMC: 2019 Sep 25.
Published in final edited form as: Nat Med. 2019 Mar 25;25(5):776–783. doi: 10.1038/s41591-019-0401-y

Extended Data Figure 10 |. Flow cytometry of CD34+ HSPCs with 24 hours of culture.

Extended Data Figure 10 |

Cryopreserved G-CSF mobilized CD34+ HSPCs were stained with CD34, CD38, CD90, and CD45RA antibodies (in a), or stained with Hoechst 33342 and Pyronin Y (in b) at 0 hours following thaw or after 24 hours in culture with SCF, TPO and FLT3-L. HSPCs were electroporated with RNP with 3x-NLS-SpCas9 with BCL11A enhancer or AAVS1 targeting sgRNA. After 2 hour recovery, cells were stained with CD34, CD38, CD90, and CD45RA or with Hoechst 33342 and Pyronin Y, and sorted according to gates as shown in c-d.