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. 2019 May 13;14(5):e0216946. doi: 10.1371/journal.pone.0216946

Fig 4. Oligomerization of TP4 peptides in the presence of sarkosyl.

Fig 4

(A) Oligomerization of TP4 peptides at various sarkosyl concentrations. TP4 peptides (4 μg) were dissolved in 10 μl of 0, 0.25x, 0.5x, 1x, 2x and 4x Sar, crosslinked with 0.1% glutaraldehyde for 30 min and subjected to SDS-PAGE. TP4 became oligomers from monomers, dimers to hexamers as shown by 1–6 although they precipitated at 0.25x and 0.5x Sar. Pellets represent the precipitates of 4 μg TP4 peptides in 200 μl sarkosyl solutions without crosslinking. (B, C) Stability of TP4 oligomers to NaCl (B) and TFE (C). TP4 peptides were dissolved in 1x Sar containing various concentrations of NaCl and TFE as indicated for 30 min before crosslinking.